Install R packages for scRNA-Seq

do not use anaconda R install biocInstaller install workflow: source("http://bioconductor.org/workflows.R") workflowInstall("simpleSingleCell")

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install apache

sudo apt-get install apache2 gksudo gedit /etc/apache2/sites-available/default sudo /etc/init.d/apache2 restart add cgi module: sudo ln -s ../mods-available/cgi.load cgi.load(mods-enable) serve-cgi-bin.conf: AddHandler cgi-script cgi py

new paper available: RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core

ArticleThe EMBO Journal advance online publication 3 September 2013; doi:10.1038/emboj.2013.198 Published online: 3 September 2013RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic coreMaria Anokhina1, Sergey Bessonov1, Zhichao Miao2, Eric Westhof2, Klaus Hartmuth1 and Reinhard Lührmann1Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Göttingen, GermanyArchitecture et … Continue reading new paper available: RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core

setup .pymolrc for pymol configuration

# one_letter["SER"] will now return "S" one_letter ={'VAL':'V', 'ILE':'I', 'LEU':'L', 'GLU':'E', 'GLN':'Q', \ 'ASP':'D', 'ASN':'N', 'HIS':'H', 'TRP':'W', 'PHE':'F', 'TYR':'Y',    \ 'ARG':'R', 'LYS':'K', 'SER':'S', 'THR':'T', 'MET':'M', 'ALA':'A',    \ 'GLY':'G', 'PRO':'P', 'CYS':'C'} set cartoon_fancy_helices, 1 set cartoon_ring_mode,1 set cartoon_ring_transparency, 0.5 set transparency, 0.5 bg_color white orient